Locate the spinal cord with the naked eye. Survey the cord under the low
power of your microscope and identify, at the surface of the cord, the
meniges(dura and arachnoid), nerve rootlets in oblique or
cross sections, dorsal root(sensory) ganglia,
blood vessels. Epidural fat
is seen. Identify the anterior(ventral) median sulcus, posterior(dorsal)
and the ependymal canal(in the centre of the cord) lined by columnar
Try to demarcate the boundary between the white and the grey matter of
the spinal cord.
Examine the grey matter under high-dry(X40)
Examine the white matter under high-dry(X40)
- Could you identify the perikarya(soma, cell bodies) of the motor
neurons in the ventral(anterior) horn?
Identify also the nucleus, nucleolus and Nissl substance in the neurons.
Arising from the perikaryon are a variable number of dendrites in short
- Between the neurons are the nuclei of neuroglail cells.
It is difficult to classify them into types in paraffin section. Review
the functions of the various neuroglial cells.
- Are the capillaries identifiable in the grey matter?
- Is the texture of the nervous tissue in the white matter identical
to that of the grey matter? How would you describe the appearance of the
white matter? The clear space between the nerve fibre and the myelin
sheath is an artefact.
- Are neuroglial cells present in the white matter?
- Do you see any blood vessels in the white matter?